detachable Elisa plate

Enzyme-linked immunosorbent assay (ELISA) plate

Product Introduction

An enzyme-linked immunosorbent assay (ELISA) plate is a consumable used with an ELISA reader for enzyme-linked immunosorbent assays, essential in ELISA testing. Made of polystyrene (PS), the plate adsorbs antigens, antibodies, and other biomolecules to its surface through mechanisms such as hydrophobic interactions and ionic reactions.


ELISA Experiment

The primary solid-phase carrier for adsorbing antigens or antibodies.

Protein Detection

ELISA Introduction

ELISA plates undergo rigorous optical density testing, making them the ideal partner for ensuring the accuracy of sensitive protein detection kits.

The basic ELISA method involves adsorbing known antigens or antibodies onto a solid-phase carrier surface, maintaining their immunological activity. These antigens or antibodies are then linked to an enzyme, preserving both their immunological and enzymatic activities.

During the assay, the test sample (containing the target antibody or antigen) reacts with the enzyme-labeled antigen or antibody on the solid-phase carrier. The antigen-antibody complexes formed are separated from other substances by washing. The amount of enzyme bound to the solid-phase carrier is proportional to the amount of the target substance in the sample.

When a substrate is added, it is catalyzed by the enzyme to produce a colored product. The intensity of the color is directly related to the amount of the target substance in the sample, allowing for qualitative or quantitative analysis. The "solid-phase carrier" in this process is the ELISA plate

Product Features

  • Made of polystyrene (PS) material.
  • Available in two binding force specifications: high binding force (300-500 ng/cm²) and medium binding force (100-200 ng/cm²).
  • Offers 8-well strips for cost-effective use with ELISA plates.
  • Flat bottom design with uniform pore size and thickness, ensuring experimental accuracy and repeatability.
  • Transparent plate body with CV value <5%, providing good inter-batch stability and high sensitivity for colorimetric assays.
  • Clear alphanumeric labeling for easy sample identification in different wells.
  • Complies with SBS international standards, compatible with most ELISA readers, and can replace similar foreign brands.
  • Free from DNase/RNase and pyrogens.


Different Binding Strengths of ELISA Plates

Medium Binding

The enzyme-linked immunosorbent assay (ELISA) plate binds passively to proteins through hydrophobic interactions on its surface, making it suitable as a solid-phase carrier for large molecular weight (>20kD) proteins. It is primarily used to immobilize large molecules with hydrophobic regions that can interact with the surface, such as antibodies. This requires a larger surface area to immobilize biomolecules, resulting in lower binding capacity (100 to 200ng IgG/cm2). Due to its adsorption mechanism, inert proteins or non-ionic detergents can easily block moderately binding surfaces. Because this surface only binds to large, hydrophobic molecules, such ELISA plates are suitable as solid-phase carriers for unpurified antibodies or antigens, reducing potential nonspecific cross-reactivity.

High Binding

Compared to medium binding surfaces, high binding ELISA plates have an increased binding capacity of approximately 300 to 500ng IgG/cm2, primarily binding proteins with molecular weights >10kD. Ionic reactions only require a small fraction of the molecule to contact the surface to achieve stable binding. Since the antigenic site (or active site) is part of the molecule with a positively charged region, there is a possibility of spatial interference when this molecule interacts with the surface. Non-ionic detergents are ineffective in blocking this surface, necessitating the addition of a protein blocking step.

Back to blog