PBMC
Peripheral Blood Mononuclear Cells (PBMCs) are a crucial component of the immune system, including lymphocytes, monocytes, dendritic cells, and other minor cells (such as hematopoietic stem cells). Widely used as the fundamental experimental material in immunology, infectious diseases, malignant tumors, vaccine development, transplant therapy, personalized medicine, and toxicology.
SmtraBio Peripheral Blood Lymphocyte Separation Tube
Compared to traditional centrifuge tubes, SmtraBio's Peripheral Blood Lymphocyte Separation Tube allows for rapid PBMC sorting. With its unique built-in separation nest design, it minimizes mixing between the target sample and the density gradient medium. After centrifugation, PBMCs are retained above the nest, separated from the red blood cells and granulocyte layer at the bottom of the tube. Collecting PBMCs only requires a simple pour, greatly reducing the experimental complexity and saving a significant amount of time.
Product Features
- 01 Rapid sorting in 15 minutes, saving experimental time.
- 02 Simple operation, no need for careful and slow sample addition.
- 03 Collecting PBMCs only requires a simple pour, reducing experimental complexity.
- 04 Good consistency, reducing operational differences between users.
- 05 Avoid recontamination by red blood cells and granulocytes.
- 06 Clearly printed scale lines on the tube body, with a blank area for easy labeling. 07 Electron beam sterilization, SAL=10^-6.
- 08 No pyrogen, no DNA/RNA enzymes.
Instructions for Use
Preparation
- Equilibrate the separation medium to room temperature (RT) and protect it from light.
- Sample preparation: anticoagulated whole blood or bone marrow. Dilute with physiological saline if necessary to improve separation efficiency.
Separation Process
- Use a pipette to add the separation medium from the center hole of the nested tube, ensuring it covers the nest: add 4-9 mL of separation medium for a 15 mL tube; add approximately 13-30 mL of separation medium for a 50 mL tube, ensuring it covers the nest.
- Slowly pour the anticoagulated sample along the tube wall or use a pipette to slowly add it along the wall of the tube.
- Centrifuge at room temperature for 10 minutes at 1200xg.
- Harvest PBMC cells by pouring the supernatant from the separation tube into another clean centrifuge tube. The nest in the separation tube effectively prevents recontamination by red blood cells and granulocytes. (Note: Before pouring out the cells after centrifugation, you can also first aspirate the top plasma layer, which helps reduce contamination by platelets.)
- Wash the PBMC cells with PBS, centrifuge at 250xg for 10 minutes.
- Repeat washing step 5 twice, resuspend the cells in 5 mL of PBS buffer.
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